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當(dāng)前位置:主頁 > 產(chǎn)品中心 > > 抗體/抗原 >Anti-Ubiquitin (linkage-specific K63) EPR8590-448 antibody 抗泛素抗體

Anti-Ubiquitin (linkage-specific K63) EPR8590-448 antibody 抗泛素抗體

簡要描述:Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody 抗泛素抗體
貨號:ab179434
廠家:Abcam公司
規(guī)格:40ul/100ul
保存溫度:-20℃ 和 2~8℃
貨期:現(xiàn)貨2~5天,期貨2-3周

  • 產(chǎn)品型號:ab179434
  • 廠商性質(zhì):代理商
  • 更新時間:2017-01-26
  • 訪  問  量:3413
詳細(xì)介紹

咨詢:  (重慶) (貴陽)

Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody 抗泛素抗體產(chǎn)品信息:

 Product nameAnti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibodySee all Ubiquitin primary antibodies

    Description

    Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63)

    Tested applicationsWB, IHC-P, ICC/IF more details

    Species reactivity

    Reacts with: Mouse, Rat, Human

    Positive control

        K63-linked-Ub2-7 recombinant protein; Human kidney carcinoma tissue.

Properties

    FormLiquid

    Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    PurityTissue culture supernatant

    ClonalityMonoclonal

    Clone numberEPR8590-448

    IsotypeIgG

    Research Areas

        Neuroscience

        Neurology process

        Neurodegenerative disease

        Alzheimer's disease

        Tangles & Tau

        Epigenetics and Nuclear Signaling

        Chromatin Modifying Enzymes

        Ubiquitylation

        Epigenetics and Nuclear Signaling

        DNA / RNA

        DNA Damage & Repair

        DNA Damage Response

        Other

        Cell Biology

        Proteolysis / Ubiquitin

        Proteasome / Ubiquitin

        Ubiquitin

        Neuroscience

        Neurology process

        Neurodegenerative disease

        Other

        Epigenetics and Nuclear Signaling

        Ubiquitin & Ubiquitin Like Modifiers

        Ubiquitin like Modifiers

Applications

Our Abpromise guarantee covers the use of ab179434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application      Notes

WB           WB: 1/1000 - 1/5000. Predicted molecular weight: 77 kDa.

IHC-P       IHC-P: 1/100 - 1/250.

ICC/IF      ICC/IF: 1/100 - 1/250.

Application notesIs unsuitable for ,Flow Cyt or IP.

Target

    RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.

    Cellular localizationCell Membrane, Cytoplasmic and Nuclear

    Database links

        Entrez Gene: 7314 Human

        Entrez Gene: 22187 Mouse

        Entrez Gene: 192255 Rat

        Omim: 191339 Human

        SwissProt: P0CG47 Human

        SwissProt: P0CG48 Human

        SwissProt: P62988 Human

        SwissProt: P0CG49 Mouse

Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody images

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with ab179434 at 1/100 dilution.

    Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

    All lanes : Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody (ab179434) at 1/1000 dilution

    Lane 1 : Ubiquitin recombinant protein

    Lane 2 : K6-linked-Ub2 recombinant protein

    Lane 3 : K11-linked-Ub2 recombinant protein

    Lane 4 : K27-linked-Ub2 recombinant protein

    Lane 5 : K29-linked-Ub2 recombinant protein

    Lane 6 : K33-linked-Ub2 recombinant protein

    Lane 7 : K48-linked-Ub2-7 recombinant protein

    Lane 8 : K63-linked-Ub2-7 recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size : 77 kDa

公司介紹:

    Abcam位于英國的劍橋科學(xué)園,成立于1998年,專門生產(chǎn)和分銷研究型抗體。主要創(chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司,理念是Abcam銷售質(zhì)量高的抗體,并提供zui全面、誠實(shí)、時新的說明書,快速的送貨服務(wù),及高效率的顧客和服務(wù)。我們的在線目錄(www.abcam.cn) 已有差不多100,000種抗體和試劑,并不斷添加,供應(yīng)予百多個國家。Abcam200511月在倫敦證券交易所上市,在美國馬薩諸塞州、日本東京及香港均設(shè)有分公司。

    Abcam 一直致力加強(qiáng)產(chǎn)品線,為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品,在2011并購了美國的MitoSciences公司,加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng);同年也并購了英國的Ascent Scientific 公司,開展了生化試劑的供應(yīng)。在2012年,Abcam并購了美國的Epitomics 公司,成為一家有的RabMAbs® 供應(yīng)商。

    Abcam 的目標(biāo)是給世界上的抗體建立zui大的在線目錄,為各地科學(xué)家提供*產(chǎn)品,成為各國科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來使客戶獲得預(yù)期的結(jié)果。為提供高質(zhì)量的抗體來指向盡可能多的靶蛋白,我們盡所能在盡可能多的應(yīng)用和物種中檢測每種抗體。我們相信誠信才是上策,在我們的上會發(fā)布有關(guān)我們每一種產(chǎn)品的盡可能多的信息。

更多產(chǎn)品:

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Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗體一抗

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更多詳情,請:     

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